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	Provedor de dados Arq. Bras. Med. Vet. Zootec. (5) BJMBR (1) Autor Cândido,A.L. (6) Resende,M. (5) Bontempo,E.D. (2) Martins,A.S. (2) Azevedo,V. (1) Barros,P.B. (1) Barros,P.P. (1) Bessa,L.R.G. (1) Cecílio,A.B. (1) Drumond,B. P. (1) Flaschart,R.B. (1) Goes,A.M. (1) Leite,R.C. (1) Levitus,G. (1) Miyoshi,A. (1) Oliveira,M.E. (1) Oliveira,S.C. (1) Mais... Palavra-chave Antigens (1) Aujeszky's disease virus (1) Aujeszky’s disease virus (1) Bovine herpesvirus 1 (1) Bovine herpesvirus-1.2a (1) Brazil (1) DNA plasmid (1) DNA vaccine (1) Dot-blot hybridization (1) ELISA (1) Enhancer/promoter region (1) Genetic immunization (1) Glycoprotein D gene (1) Immune response (1) Immunostimulatory sequences (ISS) (1) Latency (1) Mouse hepatitis virus (1) Non radioactive probe (1) ORF-1 (1) Polymerase chain reaction (1) Mais... Tipo do documento Info:eu-repo/semantics/article (5) Info:eu-repo/semantics/other (1) Ano 2003 (1) 2000 (2) 1999 (3) País Brazil (6) Idioma Inglês (6)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 6 Primeira ... 1 ... Última Detection of different Brazilian strains of the bovine herpesvirus-1 (BHV-1) by polymerase chain reaction Arq. Bras. Med. Vet. Zootec. Cândido,A.L.; Martins,A.S.; Barros,P.B.; Resende,M.. A técnica da reação em cadeia pela DNA polimerase (PCR) foi usada para a amplificação rápida de um fragmento de 456bp da região única curta (Us) do genoma do BHV-1. Iniciadores de 18pb do gene da ORF1 foram usados para a amplificação das amostras-padrão e brasileiras. Uma amplificação clássica não foi bem sucedida. A amplificação foi obtida quando se escolheu uma região com baixa concentração de GC no DNA do BHV-1 e através da desnaturação térmica (95° C para 5min) seguida de ciclos térmicos (94° C por 1min e 30seg; 52° C por 1min; 72° C por 1min e 30seg; e ainda por 35 ciclos de 94° C por 1min; 52° C por 1min; 72° C por 1min e 30seg; usando um tempo de extensão final de 72° C por 5min). A clivagem com Pst I confirmou a especificidade do fragmento da ORF 1... Tipo: Info:eu-repo/semantics/other Palavras-chave: Bovine herpesvirus 1; ORF-1; Polymerase chain reaction; Brazil. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000300007 Detection of mouse hepatitis virus in mouse colonies using the nested polymerase chain reaction Arq. Bras. Med. Vet. Zootec. Cecílio,A.B.; Cândido,A.L.; Resende,M.; Bontempo,E.D.; Martins,A.S.. A reverse transcriptase polymerase chain reaction (RT-PCR) to detect mouse hepatitis virus (MHV) in hepatic tissue was developed. To circumvent possible failures in RT-PCR amplifications, a second round of PCR with internal primers was used to confirm the specificity and increase the sensitivity of the test. Using this method specific amplification of MHV sequences was observed in 18 out of 20 mouse colonies examined. Tipo: Info:eu-repo/semantics/article Palavras-chave: Mouse hepatitis virus; RT-PCR. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400003 Development of DNA probe for detection of Aujeszky's disease virus Arq. Bras. Med. Vet. Zootec. Cândido,A.L.; Barros,P.P.; Resende,M.; Flaschart,R.B.. A DNA hybridization dot-blot assay using a radioactive and a non-radioactive probe has been developed for the detection of Aujeszky's disease virus (ADV). The Bam H I 7 fragment of ADV genomic DNA was labeled by nick translation using 32P-dCTP and the 196bp polymerase chain reaction (PCR) gG glycoprotein gene amplified fragment was also labeled by nick translation but using biotin d-7-dATP. This technique provides a fast and effective means of detecting acute cases of ADV infection but it was unable to detect ADV nucleic acid sequences in trigeminal nerve ganglia of latent infected pigs and mice. Tipo: Info:eu-repo/semantics/article Palavras-chave: Aujeszky's disease virus; Dot-blot hybridization; Radioactive probe; Non radioactive probe. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400001 Main features of DNA-based immunization vectors BJMBR Azevedo,V.; Levitus,G.; Miyoshi,A.; Cândido,A.L.; Goes,A.M.; Oliveira,S.C.. DNA-based immunization has initiated a new era of vaccine research. One of the main goals of gene vaccine development is the control of the levels of expression in vivo for efficient immunization. Modifying the vector to modulate expression or immunogenicity is of critical importance for the improvement of DNA vaccines. The most frequently used vectors for genetic immunization are plasmids. In this article, we review some of the main elements relevant to their design such as strong promoter/enhancer region, introns, genes encoding antigens of interest from the pathogen (how to choose and modify them), polyadenylation termination sequence, origin of replication for plasmid production in Escherichia coli, antibiotic resistance gene as selectable marker,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic immunization; DNA plasmid; Enhancer/promoter region; Immunostimulatory sequences (ISS); Antigens; Immune response. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200002 Partial characterization of a Brazilian strain of Aujeszky’s disease virus recovered from a pig with subclinical infection Arq. Bras. Med. Vet. Zootec. Cândido,A.L.; Resende,M.; Oliveira,M.E.; Bontempo,E.D.; Drumond,B. P.. One Brazilian strain of Aujeszky’s disease virus isolated from a piglet in which the disease had not been observed was studied as for its virulence in pigs. The genome of the virus was molecularly analysed as for their restriction endonuclease cleavage pattern. Fifty-day-old non-immune weanlings exposed to this strain showed no disease although the virus was present in their oropharyngeal area for at least three days. All animals developed moderate titers of neutralizing antibody. Based on number of bands and migration rate of restriction fragments the isolate was classified into Herrmann’s type I group. Latent infection was detected in all pigs by PCR. Some variations were detected in the cleavage pattern of the strain ASB Piau when compared to LA031... Tipo: Info:eu-repo/semantics/article Palavras-chave: Swine; Aujeszky’s disease virus; Virulence; Latency. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400001 Serological responses in sheep injected with plasmids encoding bovine herpesvirus 1 (BHV-1) gD glycoprotein Arq. Bras. Med. Vet. Zootec. Cândido,A.L.; Resende,M.; Bessa,L.R.G.; Leite,R.C.. A genetic vaccine consisting of the bovine herpesvirus-1.2a (BHV-1.2a) glycoprotein D (gD) gene under the control of the cytomegalovirus immediate-early promoter/enhancer was generated and administered to sheep intramuscularly in the neck. All animals developed serum antibodies which recognized the homologous antigen (BHV-1.2a strain BH-83) and also exhibited cross-reactivity against the heterologous antigen (BHV-5 strain EVI-190). Three intramuscularly injections were given but serological responses were not improved after the second inoculation. Specific antibodies were detected against BHV-1.2a until at least 12 months after the first inoculation. However, the capacity to induce antibodies against BHV-5 was lower and of shorter duration than to BHV-1.2a. Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine herpesvirus-1.2a; Glycoprotein D gene; DNA vaccine; ELISA. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300002 Registros recuperados: 6 Primeira ... 1 ... Última

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